By Thomas M. Bell (Auth.)
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Extra info for An Introduction to General Virology
Hoskins, M. (1935) Amer. J. Trop. Med. 15, 675. Pereira, H. G. (1960) Virology, 11, 590. Anderson, K. (1942) Amer. J. Path. 18, 577. VIRAL INTERFERENCE AND ANTI-VIRAL SUBSTANCES 51 5. Schlesinger, R. W. (1959) In "The Viruses" (F. M. Burnet & W. M. ) Vol 3, p. 157. Academic Press, New York. 6. , Hirst, G. K. (1947) /. Immunol. 57, 125. 7. Cairns, H. J. F. (1951) Brit. J. Exper. Path. 32, 110. 8. Andrewes, C. , Elford, W. J. (1947) Brit. J. Exper. Path. 28, 278. 9. , Rosenberg, E. B. (1947) /. Exper.
1941) Amer. J. Path. 17, 177. 20. Polk, A. D„ Buddingh, G. , Goodpasture, E. W. (1938) Amer. J. Path. 14,71. 2 CHAPTER FIVE TISSUE CULTURES THE history of tissue cultures is almost as old as Virology itself. Although it is nearly 40 years since Parker and Nye 1 demonstrated viral multiplication in such cultures, it is only in the last fifteen years that they have been widely used. In the early days of tissue culture, before antibiotics were discovered, stringent aseptic procedures were necessary to prevent contamination with bacteria and fungi.
Two types of glass give satisfactory results, namely, the hard, heat resistant borosilicate glass and the soft soda glass. Both may be toxic for cells when new and rigorous cleaning procedures should be followed before their initial use and subsequent re-use. Many cleaning schedules are in use. When choosing, it is wise to bear in mind the purpose of cleaning—to produce consistently good growth of cells. To date the author has found that the cheapest, easiest and most generally efficient 26 TISSUE CULTURES 27 method is the use of a good soap.
An Introduction to General Virology by Thomas M. Bell (Auth.)